Mrs. Pfahnl’s Bio A Lab: Catalase
September 29, 2020 Purpose: Investigate catalase activities in different fruits or vegetables.
Materials:
1. Hydrogen peroxide 3% from any drug store
2. Dish washing detergent any brand
3. Potato (white, yellow or sweet) or carrot 
4. Avocado 
5. Apple 
6. Tap water
7. Three narrow transparent cups labeled 1-3
8. measuring cup 
9. measuring spoon: 1 table spoon
10. ruler
11. cutting board
12. knife
Procedures:
1. Add half cup of water to each cup
2. Add one drop of dish washing detergent into each cup and mix
3. Add 1 table spoon hydrogen peroxide 3% into each cup and mix
4. cut potato/carrot, avocado and apple into 1 cm cubes
5. Add 6 cubes of potato/carrot into “cup 1”
6. Add 6 cubes of avocado into “cup 2”
7. Add 6 cubes of apple into “cup 3”
8. Measure the thickness of the foam at 3 min, 20 min
Thickness of foam at 3 min, cup 1=0.5 cm, cup 2=1 cm, cup 3 a few bubbles.
Thickness of foam at 3 min, cup 1=1 cm, cup 2=2 cm, cup 3 not much bubbles.
Observation and data analysis
1. Take pictures to document your experiment (34% of your grade)
2. Make data chart and graph for each cup. X= time Y=thickness of foam (33% of your grade)
3. Make Flipgrid video to explain the observation. Which plant material has the most catalase activity and which one has the least? (33% of your grade)